Fig. 2
From: The neurotoxicity of amyloid β-protein oligomers is reversible in a primary neuron model
Effects of Aβ-O treatment and removal on abnormal phosphorylation of tau. Primary neurons grown on coverslips were treated as in Fig. 1, followed by immunofluorescence staining with anti-total tau (a), AT8 (b), PHF1 (c) or Tau-1 (d) antibodies. Scale bar = 20 μm. e-h Immunofluorescence intensities were quantified as described in Methods, and expressed relative to those in controls on day 2 or 4. For AT8 (f), PHF-1 (g), and Tau-1 (h), immunofluorescence intensity levels were normalized to those of total tau (e). Data represent means ± SEM from three separate experiments. *p < 0.05, **p < 0.01, compared with control. #p < 0.05, compared with Aβ-O-treated cells