Fig. 4

Kainate receptor mediated EPSCs in the tree shrew. a In the presence of picrotoxin (100 μM) and AP5 (50 μM), KA receptor mediated EPSCs could be observed after application of GYKI 53655 (100 μM) and then blocked by CNQX (20 μM). Sample traces (left), sample time course points (middle), and statistical results (right) showed that the EPSCs in the presence of GYKI 53655 and CNQX (n = 6 neurons/3 tree shrews). b Representative traces of KA receptor mediated EPSCs obtained after application of different number of stimuli (1, 5, 10 and 20 shocks) at 200 Hz. c Statistical results showed that the peak amplitude of the KA EPSCs in tree shrew was larger than those in mouse by repetitive stimulations (200 Hz) (n = 11 neurons/4 tree shrews and n = 9 neurons/3 mice). Note that 5 shocks induced a saturated current. The amplitude (d) and the percentage (e) of current-voltage relationship (I-V curves from −70 to +50 mV) for KA receptor mediated EPSCs in tree shrew and mouse (n = 10 neurons/4 tree shrews; n = 7 neurons/3 mice). *P < 0.05, **P < 0.01, error bars indicated SEM