Fig. 2
From: mGluR5 regulates REST/NRSF signaling through N-cadherin/β-catenin complex in Huntington’s disease
mGluR5 modulates Src phosphorylation expression in primary neuronal cultures. Representative western blots and quantification of fold change Src phosphorylation at Y416 (pY416) with the corresponding loading controls in primary cultured corticostriatal neurons from E15 wild-type mouse embryos stimulated with either the mGluR group I agonist DHPG (100 μM) (a and b) or mGluR5-selective NAM, CTEP (10 μM) (c and d) for 10, 30, 60, 180 and 360 mins. pSrc was normalized to vinculin (n = 4 for each group). Values represent mean ± SEM and are expressed as a fraction of the non-treated (NT) cultures. * P < 0.05 vs NT cultures. Statistical significance was assessed by one-way ANOVA and Fisher’s LSD multiple comparisons