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Fig. 3 | Molecular Brain

Fig. 3

From: mGluR5 regulates REST/NRSF signaling through N-cadherin/β-catenin complex in Huntington’s disease

Fig. 3

Inhibition of either mGluR5 or Src activity increase the interaction between N-cadherin and β-catenin in primary neuronal cultures. Representative western blots (a) and quantification (b) for coimmunoprecipitation of N-cadherin with β-catenin (upper panel) in primary cultured corticostriatal neurons from E15 wild-type mouse embryos stimulated with either mGluR5-selective NAM, CTEP (10 μM), Src family kinase inhibitor A419259 (1 μM) or the mGluR group I agonist DHPG (100 μM) for 60 min. Lower panel (a) shows N-cadherin lysates used to normalize N-cadherin co-immunoprecipitation with β-catenin (n = 4 for each group). Values represent mean ± SEM and are expressed as a fraction of the non-treated (NT) cultures incubated with β-catenin antibody only. *P < 0.05 versus NT cultures. Statistical significance was assessed by one-way ANOVA and Fisher’s LSD multiple comparisons

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