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Fig. 5 | Molecular Brain

Fig. 5

From: Expression of the pacemaker channel HCN4 in excitatory interneurons in the dorsal horn of the murine spinal cord

Fig. 5

Colocalization of VGLUT2-immunoreactivity and HCN4-immunoreactivity. a, d Immunohistochemistry for VGLUT2 (green). b, e Immunohistochemistry for HCN4 (red). c, f Overlay of VGLUT2- and HCN4-immunoreactivities. Upper and lower panels are images at low and high magnifications, respectively. The areas indicated by the white boxes are expanded in lower panels. Arrowheads indicate VGLUT2-IR terminals that also show HCN4-immunostaining. Double arrowheads and arrows indicate VGLUT2-IR terminals with and without HCN4-immunostaining, respectively. Scale bars, 20 μm (upper panels) and 5 μm (lower panels)

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