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Fig. 6 | Molecular Brain

Fig. 6

From: Expression of the pacemaker channel HCN4 in excitatory interneurons in the dorsal horn of the murine spinal cord

Fig. 6

Nociceptive stimulation does not induce c-Fos in HCN4-IR cells. a Induction of c-Fos expression in the SDH by pinching. Immunoreactivities for HCN4 (red), PKCγ (green), and c-Fos (cyan). Scale bar, 200 μm. The area indicated by the white box is expanded in b. b Colocalization of HCN4- and c-Fos-immunoreactivities. b-1 Immunoreactivity for HCN4. b-2 Immunoreactivity for c-Fos. Note that c-Fos-immunoreactivity is mainly induced in lamina I. b-3 Overlay of HCN4- and c-Fos-immunoreactivity. Scale bar, 40 μm. Dashed lines indicate the borders of the PKCγ plexus. c Venn diagrams showing the relationship between the populations of HCN4-IR cells (red circle) and c-Fos-IR cells (cyan circle) in each lamina. The number shown in each category indicates the average number of cells identified in an 8-μm thick slice. d-f Induction of c-Fos expression in the SDH by thermal nociceptive stimulation. Symbols and scale bars correspond to those in a-c

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