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Fig. 1 | Molecular Brain

Fig. 1

From: AAV9-Retro mediates efficient transduction with axon terminal absorption and blood–brain barrier transportation

Fig. 1

Efficient retrograde transduction by rAAV9-Retro. a The 10-mer peptide sequence (LADQDYTKTA) from AAV2-Retro (AAV2 libraries) was inserted between Q588 and A589 of the AAV9 capsid to produce AAV9-Retro. b The AAV-CaMKIIa-mCherry and AAV-CaMKIIa-EGFP vectors were used for packaging into viruses as a specific indicator of neurons. c Transduction validation of mouse brain neurons in vivo using AAV9-Retro. The AAV-CaMKIIa-EGFP vector was packaged into the AAV9-Retro virus, and then 300 nl of rAAV9-Retro-CaMKIIa-EGFP virus was infused into the VTA region, followed by local infection. The cortical region projecting to the VTA was imaged by confocal microscopy at 21 days post-injection (DPI). Many green fluorescent signals were found in the VTA and cortex. Scale bars = 1 mm. VTA: ventral tegmental area

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Molecular Brain

ISSN: 1756-6606