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Fig. 3 | Molecular Brain

Fig. 3

From: AAV9-Retro mediates efficient transduction with axon terminal absorption and blood–brain barrier transportation

Fig. 3

rAAV9-Retro and rAAV2-Retro can retrogradely label the same projection neurons through mixed injection. a rAAV2-Retro-CaMKIIa-mCherry and rAAV9-Retro-CaMKIIa-EGFP viruses were mixed at the particle ratio of 1:1 and were injected into VTA at 300 nl. Local infection and projection regions were imaged using the Olympus VS120 Slide Scanner microscope at 21 days post-injection (DPI). B–P Both green and red fluorescent signals appeared in VTA (B–D) and in the same brain areas with projection into the VTA (E–P), including ACA, MO, PFC, AI and MSC, and most of them were overlapped. Scale bars = 200 μm. ACA: anterior cingulate area; MO: somatomotor areas; PFC: prefrontal cortex; AI: agranular insular area; MSC: medial septal complex

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Molecular Brain

ISSN: 1756-6606