Fig. 2
Correlation between microstructural changes in the brain and social behavior in Olig1-Cre/Chd8L+/F mice. a Voxel-based comparison between total contact time in the reciprocal social interaction test and FA, MD, RD, or AD values in Olig1-Cre/Chd8L+/F and Olig1-Cre/Chd8+/+ mice (n = 12 mice per genotype). Seven rostral-to-caudal axial T2-weighted structural images are overlaid with voxels that showed a significant correlation (voxel-level P < 0.01, with a cluster-level FDR correction of P < 0.05). Significant positive and negative correlations between DTI indices and behavior are represented by hot and cold colors, respectively. Two mice of the same genotype that had previously been housed in different cages were placed together in a box and allowed to explore freely for 10 min, after which the mice were subjected to an MRI scan sequence. acc and pcc, anterior and posterior corpus callosum; aCP and pCP, anterior and posterior caudoputamen; fx, fornix; PALd, dorsal pallidum; fi, fimbria; MRN, midbrain reticular nucleus; CLA, claustrum. b, c Two specific brain regions, aCP (b) and fx (c), that were associated with total contact time during the reciprocal social interaction test in a are highlighted in scatter plots. Significantly altered clusters of each brain region in a were defined as ROIs. The ROI “acc” also included motor cortex (MO) in addition to acc. The ROI “fx” also included the anterior commissure (ac) in addition to fx. The ROI “pCP” also included auditory and visceral cortex in addition to pCP. The ROI “PALd” also included CP in addition to PALd. The ROI “CLA” also included somatosensory cortex (SS) and CP in addition to CLA. The ROI “MRN” also included auditory cortex, ectorhinal cortex, and cornu ammonis 1 (CA1) in addition to MRN. Uncorrected P values are provided together with asterisks (*P < 0.05, **P < 0.01, ***P < 0.001) indicating significance level after correction by the FDR. Correlation coefficients and statistical values are also provided in Table 1. More detailed information for each cluster and statistical analysis is provided in Additional file 1: Table S1 and Additional file 2: Table S2, respectively