Fig. 3
From: Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq
A limited number of genes are impacted similarly by sleep deprivation in the transcriptome and translatome. a Venn diagram representing the number of genes differentially expressed between total RNA (microarray) and ribosome-associated transcripts (TRAP) after SD identified 49 common genes, 478 genes only in microarray and 149 genes only in TRAP study. b Chord diagram showing comparison of gene expression between TRAP and microarray data from total RNA [10]. c Quadrant scatter plots showing the fold change (log2) of the 49 common genes differentially expressed between TRAP and microarray. d Pathway analysis of the 49 common genes using PANTHER Molecular function terms showing significantly enriched pathways. All the pathways displayed have a p-value < 0.05. e, f qPCR validation from independent cohort of animals shows increased expression of Fos, Arc, Nr4A1 and Upf2, while downregulation of Cirbp in males (e) and females (f) upon sleep deprivation (SD) compared to non-sleep deprived mice (NSD). Unpaired t-test comparing SD vs NSD, males: Fos: p = 0.0002, Arc: p = 0.0011, Nr4A1: p = 0.0285, Upf2: p = 0.0010 and Cirbp: p = 0.0020, females: Fos: p = 0.0023, Arc: p = 0.0480, Nr4A1: p = 0.0016, Upf2: p = 0.0033 and Cirbp: p = 0.0005