Fig. 5

Nf1, Ctnnb1 and mTOR expression in mouse neocortical lysates. a Expression pattern of Nf1 (n = 5 WT; n = 5 Fmr1 KO), Ctnnb1 (n = 6 WT; n = 6 Fmr1 KO) and mTOR (n = 5 WT; n = 5 Fmr1 KO) in E15.5 neocortex showed no difference between the WT and Fmr1 KO mice. b Representative western blots of selected FMRP target genes. c–f Relative expression of Nf1 (n = 12 WT; n = 12 Fmr1 KO), Ctnnb1 (n = 7 WT; n = 7 Fmr1 KO), mTOR (n = 12 WT; n = 12 Fmr1 KO) and p-mTOR (n = 10 WT; n = 10 Fmr1 KO) were measured using ImageJ software. The findings indicate no difference in Nf1, Ctnnb1 and mTOR expression between WT and Fmr1 KO neocortices. However, p-mTOR at Ser2448 showed a 25.4% increase in protein level in the Fmr1 KO. Scale bars: 50 μm, 20 μm (inset). Student’s t-test was used to test statistical significance (*p < 0.05). Error bars represents the SEM