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Fig. 5 | Molecular Brain

Fig. 5

From: Altered cleavage plane orientation with increased genomic aneuploidy produced by receptor-mediated lysophosphatidic acid (LPA) signaling in mouse cerebral cortical neural progenitor cells

Fig. 5

AJ disruption enhanced non-vertical cleavage plane orientation. a Cortical sections immunolabeled for N-cadherin (red) to label neuroepithelial cells and counterstained with TO-PRO-3 (blue) to label nuclei following 2 mM EGTA treatment. E13.5 cortical hemispheres were exposed to control or 2 mM EGTA-containing medium for 10 min. EGTA-treatment disrupted N-cadherin structure at the apical side (arrows). Nuclei were counterstained with TO-PRO 3. Representative images, analyzed by confocal microscopy, of 3 paired cortices are shown. CP, cortical plate; VZ, ventricular zone; V, ventricle; scale bar, 10 µm. b Percentage of apical mitotic cells with a non-vertical cleavage plane. Data represent mean + S.E.M. (n = 3–4, *P = 0.0235, unpaired t tests)

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