Fig. 4

CRMP2-mediated decrease in sodium currents observed in DRGs from CRMP2^K374A/K374A mice is rescued in cells lacking Nedd4-2. a Representative sodium current traces recorded from small-sized DRG neurons of WT and CRMP2^K374A/K374A female mice, in response to depolarization steps from − 70 to + 60 mV from a holding potential of − 60 mV. b Summary of current density–voltage curves and c peak current density (pA/pF) from DRG neurons. Total sodium current density was significantly smaller in DRGs from homozygous female mice vs. DRG neurons from WT + siRNA-Control (n = 13 and 11 cells/condition, p = 0.0450, Kruskal–Wallis test with Dunn’s post hoc), vs. DRG neurons from WT + siRNA-Nedd4-2 (n = 13 and 15, p = 0.0314, Kruskal–Wallis test with Dunn’s post hoc), and vs. DRG neurons from CRMP2^K374A/K374A + siRNA-Nedd4-2 (n = 13 and 20, p = 0.0005, Kruskal–Wallis test with Dunn’s post hoc). d Boltzmann fits for normalized conductance G/Gmax-voltage relations for voltage dependent activation and inactivation of the sensory neurons. Half-maximal activation and inactivation (V_1/2) and slope values (k) for activation and inactivation are presented in Table 1. There were no significant differences in V_1/2 and k values of activation and inactivation between genotype and treatment (One-way ANOVA with Dunnett’s post hoc test). Error bars indicate mean ± SEM