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Fig. 3 | Molecular Brain

Fig. 3

From: Neurodegenerative phosphoprotein signaling landscape in models of SCA3

Fig. 3

Confirmation of RPMA data by Western blot analysis. For validation of RPMA data, Western blot analyses of mouse brains of SCA3 and wild-type mice were performed for selected targets. The same antibodies as in the RPMA analysis were used. Blot images (a) and graphs (b) showing their quantification. Quantified bands are marked with a black arrow. β-actin was used as loading control. In the SCA3 mouse model, transgenic expanded ataxin-3 (tg) is detected in addition to the endogenous ataxin-3 (eg). In addition, total and phosphorylated levels of AMPKβ1 and mTOR were analyzed (c and d) to further dissect the impact of expanded ataxin-3 on the mTOR signaling pathway. The bars correspond to means (wt n = 3, SCA3 n = 2–4) ± SEM. Statistical significant differences (t-test) are marked (*, p < 0.05; **, p < 0.01)

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