Fig. 1
From: A CACNA1A variant associated with trigeminal neuralgia alters the gating of Cav2.1 channels
Functional effects of the P2455H mutation on channel activation. a Schematic representation of the CaV2.1 α1 subunit with the approximate location of the Proline 2455 Histidine (P2455H) mutation in the C-terminus. b Representative calcium current traces recorded from CaV2.1α1 + 47/β4/α2δ-1 and P2455H + 47/β4/α2δ-1 channels. c Average current density–voltage relationship for cells expressing CaV2.1α1 + 47/β4/α2δ-1 or P2455H + 47/β4/α2δ-1 channels. d Normalized activation curves. Inset: mean voltage for half activation for CaV2.1α1 + 47/β4/α2δ-1 or P2455H + 47/β4/α2δ-1 channels (**p = 0.0077, two-tailed Student’s t test). e Average peak current density for whole cell calcium currents recorded from cells expressing CaV2.1α1 + 47/β4/α2δ-1 or P2455H + 47/β4/α2δ-1 channels (p = 0.8914, two-tailed Student’s t test). f Time constant of current activation (τact) as a function of voltage (*p < 0.05; **p < 0.01 vs CaV2.1α1 + 47/β4/α2δ-1, two-tailed Student’s t test). Data are represented as mean ± SEM. Numbers in parentheses represent the number of cell recordings per variant