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Fig. 6 | Molecular Brain

Fig. 6

From: Interleukin-17 induced by cumulative mild stress promoted depression-like behaviors in young adult mice

Fig. 6

Anti-IL-17 reduced increased inflammatory cytokines and microglia activation in young adult CPMS mice. Brain tissues were collected from mice after treatment with an anti-IL-17 or IgG (total 5 times for 2 weeks) in CPMS mice. Western blot analysis was performed using the cytosol isolated from the hippocampus (Hip), amygdala (Amy), and prefrontal cortex (PFC). a RORγt of Hip (CTL + Anti-IL-17; 1.307 ± 0.2092, CPMS + IgG; 1.492 ± 0.04870, CPMS + Anti-IL-17; 1.729 ± 0.1189 vs CTL + IgG), Amy (CTL + Anti-IL-17; 1.681 ± 0.8243, CPMS + IgG; 3.421 ± 0.2366, CPMS + Anti-IL-17; 3.499 ± 0.3410 vs CTL + IgG) and PFC (CTL + Anti-IL-17; 1.527 ± 0.2602, CPMS + IgG; 3.173 ± 0.1740, CPMS + Anti-IL-17; 2.656 ± 0.1406 vs CTL + IgG) was normalized by Lamin B1, and b IL-17 of Hip (CTL + Anti-IL-17; 1.228 ± 0.05834, CPMS + IgG; 1.480 ± 0.03881, CPMS + Anti-IL-17; 0.9077 ± 0.04316 vs CTL + IgG), Amy (CTL + Anti-IL-17; 1.191 ± 0.07569, CPMS + IgG; 1.373 ± 0.06446, CPMS + Anti-IL-17; 0.9261 ± 0.02749 vs CTL + IgG) and PFC (CTL + Anti-IL-17; 1.243 ± 0.04680, CPMS + IgG; 1.614 ± 0.03935, CPMS + Anti-IL-17; 0.8410 ± 0.05664 vs CTL + IgG) was normalized by GAPDH. c Immunofluorescence staining for Iba1(red) was performed in the 9-week-old mice (n = 3–5 per group). Scale bar 100 μm. Microglia intensity was quantified in Hip (CTL + Anti-IL-17; 0.9702 ± 0.06205, CPMS + IgG; 1.385 ± 0.03117, CPMS + Anti-IL-17; 1.136 ± 0.06541 vs CTL + IgG) ± Amy (CTL + Anti-IL-17; 1.017 ± 0.006536, CPMS + IgG; 1.315 ± 0.03766, CPMS + Anti-IL-17; 1.074 ± 0.9068 vs CTL + IgG), PFC (CTL + Anti-IL-17; 0.9675 ± 0.01086, CPMS + IgG; 1.403 ± 0.02017, CPMS + Anti-IL-17; 1.121 ± 0.06376 vs CTL + IgG). All data are presented as mean ± SEM of three or more independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs CTL + IgG, &p < 0.05, &&p < 0.01, &&&p < 0.001 vs CTL + Anti-IL-17, #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.001 vs CPMS + IgG

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