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Fig. 2 | Molecular Brain

Fig. 2

From: Mice with cleavage-resistant N-cadherin exhibit synapse anomaly in the hippocampus and outperformance in spatial learning tasks

Fig. 2

GD mutant N-cadherin on the plasma membrane is relatively stable. a Representative immunoblot data from cell surface protein bio-tinylation analysis for HA-tagged, WT (left) or GD mutant (right) N-cadherin expressed in CHO cells. Surface bio-tinylation pulse-labeling followed by serial immunoblot assay for the HA epitope shows that cell surface fraction of GD and WT N-cadherins decay within hours, while the levels of total N-cadherins remain unchanged. It is to be noted that the cell surface fraction of GD N-cadherin was significantly more stable than that of WT. (B) (Top) Densitometric quantification of surface biotinylation assay demonstrated that GD mutant (●) on the plasma membrane decayed significantly more slowly than WT (○). (n = 3, 3, p = 0.0241, ANOVA)

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Molecular Brain

ISSN: 1756-6606