Fig. 4

Co-immunostaining of Olig2 and NeuN in adult mouse CNS. a Sagittal overview of Olig2 and NeuN expression pattern in the mouse brain. b–m High magnification images showing the expression relationship between Olig2 and NeuN in selected brain regions. Co-localization of Olig2 and NeuN is not observed in all the regions of interest as indicated by white arrows. n Coronal overview of the mouse SC. o A high magnification image depicting the co-immunostaining of Olig2 and NeuN in the SC. Similarly, no Olig2 is expressed in NeuN⁺ cells. White arrows indicate Olig2⁺ cells without NeuN co-localization. Scale bars: 1000 μm (a), 50 μm (b–m, o), 500 μm (n)