Fig. 3

PDK1 knockdown promotes axon regeneration in vitro. a In vitro axon regeneration assays. Mouse embryonic DRG neurons were re-plated, fixed, and immunostained with TUJ1 antibody. Control, shRNA-control lentivirus and PDK1 KD, shRNA to PDK1 lentivirus. Scale bar, 100 μm. b Average length of the regenerating axons from (a) (Mean ± S.E.M.; n = 331 and 374 cells for each condition; ***p < 0.001 from a t-test). c Relative mRNA levels measured using quantitative RT-PCR from cultured embryonic DRG neurons to confirm the successful knockdown of PDK1 (Mean ± S.E.M.; n = 3; ***p < 0.001 by t-test). d Western blot analysis of protein lysates from cultured embryonic DRG neurons from shControl or PDK1 KD samples