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Fig. 1 | Molecular Brain

Fig. 1

From: Long-range GABAergic projections from the nucleus of the solitary tract

Fig. 1

Long-range projections of NTS GAD2 neurons. a Horizontal view of the whole-brain projections from NTS GAD2 neurons. Brain regions labeled by dotted lines indicate the AAV injection site in the NTS and terminal-targeted regions in the BST, PVH, VP, PSV and SPV. A, anterior; P, posterior; L, left; R, right; D, dorsal; V, ventral. a1 Coronal section at the position in (a) showed infections of AAV-DIO-eGFP in the NTS, and axonal projections in the nearby regions such as the SPV, PARN, IRN and medullary reticular nucleus (MDRN); boxed regions are magnified to show the details (a2-a3). bd Maximum-intensity projections of coronal sections showing axonal terminals originating from NTS GAD2 neurons in the SPV (b), BST (c) and PVH (d). Images are maximal projections of 128-μm z-stacks. b1, c1, d1 Magnification of axonal boutons in the SPV (b1), BST (c1) and PVH (d1) at the frames indicated in (b), (c) and (d), respectively. b2, c2, d2 Sagittal view of the frames indicated in (b1, c1, d1). e, g Representative images showing expressions of tdTomato fluorescence in GAD2 neurons and CTB-AF488 signal in neurons retrogradely traced from the BST (e1e3) and PVH (g1–g3). Images are maximal projections of 100-μm z-stacks. f, h Magnified views of the boxed areas in e and g showing colocalizations of CTB and tdTomato signals. Arrows indicated GAD2-positve CTB neurons. i, j Fluorescent somas in the NTS indicated GAD2 neurons projecting to the BST (i) and PVH (j). i1j1 Magnification of the frames indicated in the (i) and (j). Arrows indicated GAD2-positve neurons. Images are maximal projections of 100-μm z-stacks. k Horizontal and sagittal view of reconstruction of individual short-range and long-range neurons

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