Fig. 1

Increased ribosomal protein levels, protein synthesis, and ERK activity in the striatal synaptosome of Shank3-overexpressing transgenic (TG) mice compared with wild-type (WT) mice. a The graph shows relative protein abundance between the TG and WT striatal synaptosomes. The six ribosomal proteins (RPs) up-regulated in TG mice and their fold-change values are indicated. b Western blot images and graph showing the expression levels of RPs in whole lysate and synaptosome of the TG striatum compared with the WT striatum (n = 6–7 mice per genotype). c Western blot images and graph showing the levels of puromycin (Puro.) labeling in whole lysate and synaptosome of the WT and TG striatal slices (n = 7 mice per genotype). d Western blot images and graph showing the levels of total and phosphorylated mTOR and ERK in the striatal synaptosome of WT and TG mice (n = 8–10 mice per genotype). e Western blot images showing the distribution of the RPs, RPS6 and RPL36A, in different sucrose density-gradient fractions of the WT and TG striatal synaptosome. Samples from WT liver were loaded as a positive control for sucrose density-gradient fractions. f Graph showing the results of qRT-PCR validation for the enrichment of CaMKIIa and Dlg4 mRNAs in polysome-enriched fractions of the striatal synaptosome. g Schematic diagram showing the multi-omics datasets of the WT and TG striata. h–j Graphs showing the comparisons of fold-change values between the proteomic change and each of the transcriptomic changes in the TG striatum. k Graphs showing the comparisons of fold-change values between the top 50 up-regulated proteins and synaptic polysome-associated transcripts (upper panel), as well as between the top 50 down-regulated proteins and synaptic polysome-associated transcripts (lower panel) in the TG striatum. l Schematic diagram showing a hypothesis that Shank3, via the ERK pathway, regulates synaptic protein synthesis, which provides proteins to the postsynaptic density (PSD). Data are presented as the mean ± SEM. *P < 0.05 (unpaired two-tailed Student’s t-test). All raw image and quantification data for Western blotting is provided in Additional files 3 and 4