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Fig. 1 | Molecular Brain

Fig. 1

From: SCAMP5 mediates activity-dependent enhancement of NHE6 recruitment to synaptic vesicles during synaptic plasticity

Fig. 1

Forskolin-induced cLTP enhances presynaptic activity in cultured hippocampal neurons. a Experimental procedure of imaging and analyzing FM 1–43 uptake and destaining after cLTP induction by forskolin or DMSO application. b Representative images of cultured hippocampal neurons at DIV14 during FM 1–43 destaining in control (− cLTP) and cLTP-induced (+ cLTP) neurons. Scale bar: 10 μm. c Average fluorescent intensity of FM 1–43 during destaining period with or without cLTP induction. Traces of individual response (light gray lines) and average trace (thick colored lines) of the FM 1–43 signal at presynaptic boutons after treatment of DMSO (− cLTP, n = 170 boutons in 6 coverslips) or forskolin (+ cLTP, n = 167 boutons in 6 coverslips). d Turnover extent of FM 1–43 during destaining period. − cLTP, 463.629 ± 51.637 a.u., n = 6 (coverslips); + cLTP, 800.752 ± 61.040 a.u., n = 6 (coverslips), p = 0.002, analyzed by Student’s t-test. e Normalized average fluorescent intensity of FM 1–43 during destaining period with or without cLTP induction. f Time constant (τ) of FM 1–43 intensity during destaining period. − cLTP, 26.871 ± 2.054 s, n = 6 (coverslips); + cLTP, 16.543 ± 0.737 s, n = 6 (coverslips), p = 0.0008, analyzed by Student’s t-test. g Representative images of cultured hippocampal neurons co-transfected with DsRed and GFP-synaptophysin (SYP) with or without cLTP induction. Scale bar: 5 μm. h The number of presynaptic boutons per 10 μm axon. − cLTP, 3.179 ± 0.242 counts/10 μm, n = 6 (coverslips); + cLTP, 4.594 ± 0.411 counts/10 μm, n = 6 (coverslips), p = 0.021, analyzed by Student’s t-test. Values are indicated as mean ± SEM. a.u., arbitrary unit. *p < 0.05, **p < 0.01, ***p < 0.001

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