Fig. 1

Cav1 co-localizes with lipid rafts at nerve terminals in primary cultured hippocampal neurons. a Representative images of exogenous synaptophysin-GFP (left), a nerve terminal marker, together with exogenous Cav1-mCherry (Cav1-mCh; middle) in primary cultured hippocampal neurons. Neurons were co-transfected with synaptophysin-GFP and Cav1-mCh at DIV8 and fixed at DIV14-18. b Correlation between synaptophysin-GFP and Cav1-mCh expression levels. c Representative images of synapsin I and lipid rafts (CT-B) in primary cultured hippocampal neurons. Neurons were stained with Alexa-Fluor 594–conjugated CT-B (red) and its antibodies, and subsequently immunostained using an anti-synapsin I (green) antibody (SYSY) and Alexa-labeled secondary antibody (Alexa-488). d Correlation between synapsin I and CT-B expression levels. Scale bar: 10 μm (up) and 2 μm (bottom)