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Fig. 3 | Molecular Brain

Fig. 3

From: The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex

Fig. 3

Conserved interactions between TMEM132 and WRC in human and C. elegans. a Yeast colony growth indicating interaction between protein C-termini from human TMEM132D and NAP1, as well as C. elegans TMEM-132 and GEX-3. Both library-derived and re-cloned NAP1 cDNAs showed specific interaction with TMEM132D. BD and AD refer to bait/prey vectors as controls. b Schematic of human and C. elegans homologs encoding various major components of WRC that controls actin nucleation via the ARP2/3 complex. c Exemplar Western blot showing biochemical interaction between V5-tagged TMEM132D and GFP-tagged NAP1 in CoIP assay. Mammalian expression vectors encoding TMEM132D-V5 and GFP-tagged WRC components were co-transfected to HEK293 cells followed by GFP-Trap CoIP and blotting with antibodies against V5. Only NAP1 and SRA1 showed robust interaction with TMEM132D. d Exemplar confocal immunofluorescence images showing co-localization of GFP-tagged TMEM132D and mCherry-tagged NAP1 in iPSC-derived human neurons transfected with both fluorescence reporters. Scale bar, 10 µm

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