Fig. 4

C-terminal domain of SGTA interacted with aggregates in HD model cells. a Schema of the full-length SGTA and deletion mutant constructs. b Immunocytochemistry of HD150Q cells transfected with expression vector of full-length SGTA or deletion mutants. Each expression protein was tagged with V5 protein and labeled with anti-V5 antibody. Scale bar = 20 μm. Ratio of SGTA colocalization with polyQ aggregates is also shown. c Filter trap assay for comparing aggregate formation in SGTA-, LacZ- (negative control), and Hdj1- (positive control) overexpressing cells. HD150 cells were transfected with each vector and cell lysates were subjected to filter trap assay. Experiments were performed three times and statistical significance was assessed by Student’s T-test