Table 1 Screening of control and PRKN-mutated TH-GFP iPSC lines by PCR analysis for TH-GFP allele
Original iPSC line | Target site | No. of RFP-positive colonies | No. of colonies with TH-GFP allele |
|---|---|---|---|
Control (201B7) | Target 1 | 6 | 1 |
Target 2 | 5 | 0 | |
Control (WD39) | Target 1 | 3 | 3 |
Target 2 | 6 | 2 | |
PRKN (PB2) | Target 1 | 6 | 4 |
Target 2 | 9 | 0 |