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Fig. 2 | Molecular Brain

Fig. 2

From: Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors

Fig. 2

Cr ablation in L5a impairs the organization of barrels. a–d Immunostaining of CR and vGlut2 showing the distribution of CR+ L5a pyramidal neurons and barrels. During the development of the barrel cortex, CR+ L5a pyramidal neurons displayed a distinct serrated alignment pattern (high-magnification views of the boxed regions), and their dendrites extended towards the septa (white arrows: the dendrites formed septa-like structures). At P4 (a), the pattern was distinct. The peak expression level of CR was observed at P8 (b), and the pattern became more distinct and was maintained until P15 (c). The expression of CR in L5a pyramidal neurons was indistinct at P30 (d), and the pattern almost disappeared. e Immunostaining of vGlut2 in the P8 barrel cortex showed that the organization of the barrels was disordered in Cr KO mice (indicated by the red arrows; Cont, n = 4; KO, n = 4). f Nissl staining at P8 showing that, in contrast to the clear thin barrel wall observed in the control barrel cortex, abnormally gathered spiny stellate cells were observed in L4 of the Cr KO cortex (indicated by the white arrowheads; Cont, n = 4; KO, n = 4). Scale bars: a–d 200 μm; e 500 μm; f 100 μm

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