Fig. 1

Whole-tissue Fos⁺ Immunolabeling of Adult Brains by iDISCO + method. a Expression of Fos-like immunoreactivity along the entire length of the anterior-posterior (A-P) axis of the insular cortex after intraperitoneal injection of LiCl (15 mg/kg). The left panel in each column was the reference annotation image while the right was the raw data of optical sections of lightsheet imaging. Totally, 6 representative segments of the insular cortex were selected and shown (Bregma 2.0 mm, 1.42 mm, 0.98 mm, 0.26 mm, − 0.15 mm, − 0.94 mm). b Panels are higher-magnification images of insular cortex along the A-P axis. c Scatter plot displaying the density of Fos⁺ immunolabeling neurons in the insular of adult mice (n = 6 mice per group, Data are presented as means ± SEM)