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Fig. 2 | Molecular Brain

Fig. 2

From: Drosophila Graf regulates mushroom body β-axon extension and olfactory long-term memory

Fig. 2

Mutation in Graf causes MB β lobes to over-extend beyond the brain midline. a-d Confocal z-projections of adult brains immunostained with anti-FasII antibody, which strongly labels the α and β lobes and weakly labels the γ lobe. a Wild-type (WT) brain with normal MB morphology. The lateral β and γ lobes from both hemispheres terminate near the brain midline without crossing it. b-d Graf mutant (Graf1/Df) brains exhibiting mild (b), moderate (c), and severe (d) levels of midline crossing by the β lobes (arrows). A mild phenotype was defined as a thin band of axon fibers crossing the midline; a moderate phenotype was defined as a substantial fiber bundle crossing the midline that was narrower than the width of the β-lobe termini; a severe phenotype was defined as a densely stained bundle crossing the midline that was equal to or greater in width than the adjacent β lobes [31]. e Quantification of the β-lobe midline-crossing phenotype in wild-type, Graf1/Df, Graf1,C155-GAL4/Df, + ; + /UAS-Graf-HA, Graf1/Df; + /UAS-Graf-HA; OK107-GAL4/ + , and Graf1/Df; + /UAS-OPHN1-HA; OK107-GAL4/ + adult flies (n = 30 brains). Arrowheads indicate the brain midline. Scale bar, 50 μm

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