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Fig. 4 | Molecular Brain

Fig. 4

From: Drosophila Graf regulates mushroom body β-axon extension and olfactory long-term memory

Fig. 4

The β-lobe midline-crossing phenotype in Graf mutants develops during pupal development. Representative confocal z-projections of anti-FasII-labeled α and β lobes from wild-type and Graf1/Df pupae at indicated stages (n = 24 brains). a At 24 h APF, the medial β lobes extend toward the brain midline (arrowheads) in both wild-type and Graf mutant flies. At this developmental stage, the α and β lobes are very thin compared with their mature morphology. b At 36 h APF, β-lobe termini project onto the ellipsoid body (EB) without crossing the brain midline in the wild-type fly, whereas β-lobe fibers in the Graf mutant brain have crossed the midline (arrow). c At 48 h APF, MB lobes have thickened, and the β-lobe midline-crossing phenotype (arrow) can be seen more clearly. The insets show magnified regions of the midline. Scale bar, 50 μm

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