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Fig. 6 | Molecular Brain

Fig. 6

From: Drosophila Graf regulates mushroom body β-axon extension and olfactory long-term memory

Fig. 6

Genetic interactions for the β-lobe overextension defect between Graf and EGFR signaling pathway components. a Western blot analysis of brain lysates prepared from wild-type and Graf1/Y adult brains, using anti-pERK and anti-ERK antibodies. b Normalized ratios of pERK to ERK from three separate blots (**P < 0.01; Student’s t test). c Single confocal sections showing the pERK immunoreactivity in the MB neurons of UAS-NLS-mCherry/ + ; OK107-GAL4/ + (wild type) and Graf1/Y; UAS-NLS-mCherry/ + ; OK107-GAL4/ + (Graf1/Y) adult brains. Scale bar, 20 μm. di Sample confocal z-projections of anti-FasII-stained adult brains in OK107-GAL4/ + (d), UAS-EGFRCA/ + ; OK107-GAL4/ + (e), Graf1/Y (e), Graf1/Y; Egfrf24/ + (g), Graf1/Y; rl1/ + (h), and Graf1/Y; rl1/rl10 (i) flies. Arrowheads indicate the brain midline. Note that reduction or loss of EGFR signaling pathway components suppresses β-lobe overextension (arrows) in Graf mutant brains. Scale bar, 50 μm. j Quantification of the β-lobe midline-crossing phenotype in adult brains of indicated genotypes (n = 30 brains)

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