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Fig. 1 | Molecular Brain

Fig. 1

From: FMRP attenuates activity dependent modifications in the mitochondrial proteome

Fig. 1

modified by treatment and/or genotype. Most protein changes occur after TTX-APV treatment irrespective of genotype. g Heat map of the normalized Log2 protein expression levels highlighted in panel (f) that compare wild type and Fmr1−/y cultures (14 hits) and wild type and Fmr1−/y cultures after TTX-APV treatment (23 hits). h Fmr1−/y neurons incubated with TTX-APV for 48 h significantly increase the number of hits as compared to TTX-APV-treated wild type neurons. P value and Z score analysis of the number of hits in (f). X and Y axes numbers represent the number of hits in parentheses in (f). Compare wild type TTX-APV (159) to Fmr1−/y TTX-APV (260), p < 0.0002 and z-score 5. Circle size and color denote p or z-score values. Values calculated with the Vassar’s Difference between Two Independent Proportions tool. i Venn diagram all proteins quantified by TMT overlapping with curated the FMRP target mRNAs curated by Suhl et al. 2014. See Additional file 1: Table S1

The neuronal proteome sensitive to activity deprivation and Fmr1−/y. a Diagram of the experimental design. b Representative Coomassie and FMRP-beta actin blots of cultured neuronal extracts. c Volcano Plots of TMT mass spectrometry of wild type and Fmr1−/y neurons (DIV12) incubated in vehicle or TTX-APV for 48 h. N = 6 wild type and n = 4 Fmr1−/y for vehicle treated cultures, n = 5 cultures for each genotype treated with TTX-APV. The TMT labeling was performed once after all samples were collected. Blue symbols represent down-regulated proteins, red symbols upregulated proteins. FMRP is marked by dark blue and crossed square symbol. Yellow symbols depict mitochondrial proteins significantly changed. d Principal component analysis of the 6074 TMT protein quantifications. Note close grouping of all conditions. e Similarity matrix of wild type and Fmr1−/y neurons incubated in the presence of vehicle or TTX-APV for 48 h. Kendall Tau clustering analysis. Note the clustering based on TTX-APV treatment. f Venn diagram of common hits in wild type and Fmr1−/y neurons incubated in vehicle or TTX-APV for 48 h. Parentheses represent total number of proteins significantly

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