Skip to main content
Fig. 1 | Molecular Brain

Fig. 1

From: Activation of acid‐sensing ion channels by carbon dioxide regulates amygdala synaptic protein degradation in memory reconsolidation

Fig. 1

Inhibition of ubiquitination prevents CO2 and retrieval-induced AMPAR- EPSC rectification. a Experimental procedure of aversive conditioning (Av. C.), memory retrieval (Ret), amygdala slices collection, and patch-clamp recording. On day 1, the mice were subjected to 6 pure tones, paired with 6 foot shocks in context A. One day after, 50µM Ziram or saline was injected into the amygdala bilaterally and then returned to their home cages. One hour later, the mice were placed in context B and subjected to one pure tone as retrieval. 10 min after retrieval, the mice were euthanized, and the amygdala slices were collected and incubated in ACSF for patch-clamp recordings. b Percentage of freezing time during the CSs in aversive conditioning. Percentage of freezing time during the CSs in memory retrieval. d AMPAR-EPSC current-voltage relationship in recorded neurons in saline and Ziram injection groups. Insets show an example of the AMPAR-EPSCs in − 80 mV and + 60 mV in each group. e Effects of Ziram on the AMPAR-EPSC rectification index (current at − 80 mV / current at + 60 mV) in the WT and the ASIC1a−/− mice groups. f Application of 50 µM NASPM on the AMPAR-EPSCs. D-APV (100 µM) was added to the bath solution throughout the experiments to block NMDA currents and Picrotoxin (100 µM) was added to the ACSF throughout the recordings to avoid inhibitory responses. g Relative NASPM-sensitive currents, average of the last 5 recordings in each group (% of baseline) were compared in the Ziram and saline injection groups. Data are mean ± SEM. n = 8–10 cells in each group. p values are indicated in each panel, by nonparametric unpaired Mann–Whitney test

Back to article page