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Fig. 6 | Molecular Brain

Fig. 6

From: A role for BDNF- and NMDAR-induced lysosomal recruitment of mTORC1 in the regulation of neuronal mTORC1 activity

Fig. 6

Optogenetic activation of mTORC1 in neurons. Hippocampal neurons were transfected and photoactivated as in Fig. 5. A Neurons were bathed either in ACSF or in conditioned medium (CM), and processed for P-pS6 immunofluorescence 25 min or 85 min after the end of photoactivation (PA), as indicated. Photoactivation increased P-pS6 in CM (N = 45 neurons per condition from 3 independent experiments) but not in ACSF (N = 30 neurons per condition from 2 independent experiments). Images of representative neurons for each condition are shown on the right. B Synergistic activation of mTORC1 by photoactivation and BDNF or insulin. Neurons were bathed in CM containing 500 nM TTX and 50 µM AP5 to reduce basal levels of mTORC1 activity. Under these conditions, without photoactivation, BDNF or insulin had no significant effect on P-pS6 by themselves. Photoactivation triggered an increase in P-pS6 when it was combined with BDNF or insulin treatment (N = 60 images per condition from two independent experiments in both conditions). Images of representative neurons for each condition are shown on the right. Bar, 10 µm

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