Fig. 2

Reshuffling of the oG. a Various G genes used in this study. The G gene was located at the region between the M gene and L gene in the genome of RV. The B19G is a wild type G gene of RV SAD B19 strain [3], the oG is a codon-optimized chimeric version [15], and ooG is a rearranged gene based on the mouse CPB in this study. b Each salmon red circle represents a CPBS of a single mouse gene plotted against its gene length. The average CPBS of mouse is 0.06508. The carolina blue dot and the violet dot represent the oG and ooG genes, respectively. The average CPBSs of the oG and ooG are − 0.0738 and 0.3114, respectively. c Characteristics of the oG and ooG genes. The reshuffled oG (ooG) is possible to optimize the ability of protein expression in mouse, while the oG might be deoptimized in mouse