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Fig. 6 | Molecular Brain

Fig. 6

From: Meningitic Escherichia coli α-hemolysin aggravates blood–brain barrier disruption via targeting TGFβ1-triggered hedgehog signaling

Fig. 6

E. coli α-hemolysin was responsible for the infection-caused decrease of Gli2 and ZO-1 as well as the BBB integrity damage. A The expression of ZO-1 and Gli2 in hBMECs in response to RS218-WT, RS218-ΔhlyA, and RS218-ΔhlyA-phlyCA strains, as well as other α-hemolysin operon genes mutants. B Mice survival upon the infection of RS218-WT, RS218-ΔhlyA, and RS218-ΔhlyA-phlyCA strains (n = 10). Survival data were collected and shown as Kaplan–Meier survival curves, and the statistical analysis was carried out by Log-rank (Mantel–Cox) test. **p < 0.01. ns, no significance. C Evan’s blue and IF assays showing the BBB permeability and both Gli2 and ZO-1 expression in brains of mice challenged by RS218-WT, RS218-ΔhlyA, and RS218-ΔhlyA-phlyCA strains. The white triangles indicated Gli2 in BMECs. Scale bar indicated 50 μm

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