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Fig. 4 | Molecular Brain

Fig. 4

From: Cirbp-PSD95 axis protects against hypobaric hypoxia-induced aberrant morphology of hippocampal dendritic spines and cognitive deficits

Fig. 4

Effects of over-expression of Cirbp in primary hippocampal neurons exposed to hypoxia on PSD95 expression and the spine morphology. A, B The protein levels of Cirbp and PSD95 in Lenti-EGFP Con and Lenti-EGFP Cirbp infected primary hippocampal neurons (A) and HT-22 cells (B) (n = 3 biological replicates, two-way ANOVA, ± SEM). C Fluorescence images of EGFP Cirbp and mCherry-3FLAG lentivirus infected primary hippocampal neurons under normoxia or hypoxia exposure, scale bar = 10 μm. D The zoomed picture of dendritic spines, corresponds to the in the white frame of via laser confocal microscopy (white triangle indicated filopodia and yellow triangle indicated the site of spine formation, scale bar = 5 μm). E The statistical analysis of dendritic spine density (6 neurons/3 mice per group, two-way ANOVA, ± SEM). *p < 0.05

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