Skip to main content
Fig. 10 | Molecular Brain

Fig. 10

From: Overexpression of LIMK1 in hippocampal excitatory neurons improves synaptic plasticity and social recognition memory in APP/PS1 mice

Fig. 10

Overexpression of LIMK1-EGFP improves social memory in 3-month old APP/PS1 mice. A Normal social interaction during stage 2 of the three-chamber social test in APP/PS1 mice expressing EGFP or LIMK1-EGFP (APP/PS1 + EGFP n = 11, p < 0.001; APP/PS1 + LIMK1-EGFP n = 13, p < 0.001; two-tailed paired t-test). B Preference for S2 over S1 during stage 3 of three-chamber social test in APP/PS1 mice expressing LIMK1-EGFP but not in APP/PS1 mice expressing EGFP (APP/PS1 + EGFP n = 11, p = 0.178; APP/PS1 + LIMK1-EGFP n = 13, p < 0.001; two-tailed paired t test). C Discrimination scores during stage 3 of the three-chamber social test showing significantly improved social memory in APP/PS1 mice expressing LIMK1-EGFP compared to APP/PS1 mice expressing EGFP (p = 0.002, two-tailed t-test). D Both APP/PS1 + LIMK1-EGFP and APP/PS1 + EGFP mice showed memory acquisition during trials 1–5 of the five-trial social memory assay (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14; genotype: F(1,11) = 0.027, p = 0.873; trial: F(4,44) = 59.429, p < 0.001; repeated two-way ANOVA). On trial 6, APP/PS1 + LIMK1-EGFP mice showed significantly more interaction time during the presentation of a novel stranger mouse compared to APP/PS1 + EGFP mice (p = 0.001, two-tailed t-test). E Open field test showing travel distance in APP/PS1 mice expressing EGFP or LIMK1-EGFP (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.393, two-tailed t-test). F Average travel speed in pen field test (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.380, two-tailed t-test). G Time spent in center arena in open field test (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.371, two-tailed t-test). H Time spent in peripheral arena in open field test (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.474, two-tailed t-test). I Travel distance in elevated plus maze test (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.543, two-tailed t-test). J Time spent in closed arms in elevated plus maze test (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.725, two-tailed t- test). K Time spent in open arms in elevated plus maze test (APP/PS1 + EGFP n = 12, APP/PS1 + LIMK1-EGFP n = 14, p = 0.818; two-tailed t-test). Data are presented as mean ± s.e.m. n = number of mice. **p < 0.01, ***p < 0.001, ns = not significant

Back to article page