Fig. 5

The phosphorylation of cav-1 regulates lipid rafts dependent endocytosis. a Each cell line was incubated with 50 nM BODIPY FL C5-Lactosylceramide (LacCer) (Green) and 2.5 μg/ml rhodamine-conjugated transferrin (Red) for 5, 15 and 30 min. b Each cell line was pretreated with 10 μM SKI-1 or 0.1 μM saracatinib for 30 min and further incubated with 50 nM BODIPY FL C5-Lactosylceramide (LacCer) (Green) and 2.5 μg/ml rhodamine-conjugated transferrin (Red) for 20 min. Next, the cells were fixed and observed via confocal microscopy. The intensity of five (a) or four (b) independent experiments were quantified. Blue indicates DAPI staining. Scale bars indicate 20 μm. *p < 0.05, ***p < 0.001, two-way ANOVA