Fig. 4
From: Altered hippocampal transcriptome dynamics following sleep deprivation
RT-qPCR validation of RNA-Seq results. From an independent cohort of mice (n = 6 in each group), RT-qPCR was used to validate the findings of chosen genes. a Four genes related to RNA binding proteins and/or splicing: Cirbp (P-value = 1.9 × 10–3), Srsf7 (P-value = 1.6 × 10–3), Tra2a (P-value = 3.0 × 10–3), and Upf2 (P-value = 0.0168); b three genes related to transcriptional activity: Nfil3 (P-value = 0.0388), Nr4a1 (P-value = 1.0 × 10–4), and Erf (P-value = 2.6 × 10–3); c four genes related to cellular signaling: Pdgfrb (P-value = 3.2 × 10–3), Dusp5 (P-value = 2.0 × 10–4), Dusp6 (P-value = 2.0 × 10–3), and Ackr3 (P-value = 2.8 × 10–3); and d two genes related to cytoskeleton: Filip1 (P-value = 2.1 × 10–3) and Arc (P-value < 0.0001). Data are presented as mean ± SEM and normalized against two housekeeping genes (Tubulin and Hprt). Differences are significant at * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P < 0.0001, and evaluated using an unpaired t-test