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Fig. 3 | Molecular Brain

Fig. 3

From: Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply

Fig. 3

C10 is preferred over C8 as a metabolic substrate in brain slices. Competition assay between [U-13C]C8 + unlabeled 12C10 and [U-13C]C10 + unlabeled 12C8 in mouse cerebral cortical slices (molecular carbon labeling, MCL, relative to the MCL without competing 12C fatty acids (control)). All MCFAs were provided at concentrations of 200 µM in addition to 5 mM d-glucose. Metabolism of an unlabeled substrate (12C) will dilute the 13C accumulation and hereby lead to a reduction in the MCL. C8: octanoic acid. C10: decanoic acid, GAD: glutamate decarboxylase, GS: glutamine synthetase. Mean ± SEM, n = 6 from individual animals, repeated measures 1-way ANOVA with Bonferroni post hoc test, *p < 0.05

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