Fig. 2

The scheme of event detection. The process of event detection consists of four steps. The asterisk represents the region of the event shown at an extended timescale in the right panel of each row. a, b Raw traces were smoothed using an S–G filter. c Any valley point below the amplitude threshold (3 pA, dashed blue line) considered as a local minimum (red plus symbol). d The baseline of a local minimum was found using a backward search algorithm (cyan horizontal bar). e The final event (red x symbol) was determined by comparing the amplitude of the event with the minimum amplitude that a user established (dashed red line). In (c, d), the green line indicates the trend of trace