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Fig. 3 | Molecular Brain

Fig. 3

From: Minhee Analysis Package: an integrated software package for detection and management of spontaneous synaptic events

Fig. 3

Validation of the event detection algorithms using simulated data. a Artificial EPSCs were randomly generated by the multiexponential equation (Eq. 1). To generate various kinetics of EPSCs, the rise and decay time constants (τ) were randomly scaled by multiplying them with a random factor (mean 0, standard deviation 0.3). The individual and averaged traces of the simulated EPSCs are in gray and red, respectively. b White noise was generated according to the normal distribution, and then added to the idealized waveform (top, idealized trace; bottom, noise-added trace). c Representative traces of the simulated EPSC generated with three levels of background noise. The standard deviations (SD) of noise were 2 pA (top, black), 6 pA (middle, red), and 10 (bottom, blue), respectively. d A histogram showing the amplitude of the EPSC detected in the simulated traces. For SD of noise = 2 and 6 pA, the adjustable parameters were as follows: Minimal amplitude = 10, Polynomial order = 3, Side points = 14. For SD of noise = 10 pA, while the Polynomial order was the same as others, the Minimal amplitude and Side points were adjusted to 15 and 20, respectively, to minimize the number of false-positive events

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