Fig. 2

Chronic inflammatory pain-induce anxiety rat increased the excitability of ACC PNs A Representative images of c-Fos positive cells (red) and CaMKII (red) positive cells in the bilateral ACC of Control and Model group. The number of c-Fos positive cells and CaMKII positive cells per 0.1 mm² in the ipsilateral ACC (B) and contralateral ACC (C) of Control and Model group. D Schematic diagram depicting the anatomical localization in electrophysiological recording and the morphological property of pyramidal neurons. n = 3–4. *P < 0.05, **P < 0.01, compared to the Control group, Bar = 50 μm. E The firing phenotypes of PNs in ACC layer V in Control and Model groups G The firing rates in response to prolonged depolarizing current injections of increasing amplitudes in control and model groups. The input resistance (H), AP threshold (F) and resting membrane potentials (I) of PNs in ACC layer V were statistically analyzed in control and model groups. n = 9 or 12 neurons from 3 or 4 rat/group. *P < 0.05, **P < 0.01, compared to the Control group. All data represent the mean ± SEM