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Fig. 7 | Molecular Brain

Fig. 7

From: Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro

Fig. 7

Phenotypic analysis of hiPSC-neurons on day 49 of differentiation. a Immunofluorescence analysis of glutamatergic neurons (VGLUT1 and VGLUT2), dopaminergic neurons (TH), GABAergic neurons (GAD67), cholinergic neurons (VAChT and ChAT), and glial cells (GFAP) on day 49 of differentiation in 1210B2 cells. The nuclei were stained with DAPI (blue). Scale bar: 20 μm. b, c Comparison of the gene expression levels of VGLUT1 (b) and VGLUT2 (c) in hiPSC-derived neurons (1210B2 and 1201C1, both without NGF) on day 49 and in adult human samples (cerebellum and cerebrum). UD undetermined

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