Fig. 1From: Nanoscale organization of Nicastrin, the substrate receptor of the γ-secretase complex, as independent molecular domainsSubsynaptic compartmentalization of Nicastrin within functional zones of an excitatory synapse using STED microscopy. A–C Nanoscale distribution of Nicastrin (magenta) in pre/post/perisynapse with pseudocolour overlay of a presynaptic marker for the cytomatrix at the active zone (CAZ) in A, a postsynaptic marker for postsynaptic density (PSD) in B and a perisynaptic marker for the endocytic zone (EZ) in C i.e., Bassoon, Shank2 and Dynamin (green), respectively. Overlap between functional zones of the synapse and Nicastrin is presented in black. (1, 2, 3, 4, 5, 6) are magnified insets of regions indicated in A–C. Scale bar in A–C indicates 2.25 μm (left) and 300 nm (right, inset). D, E Indicate the distribution of the length D and intensity E of Nicastrin nanodomains in pre/post/perisynaptic compartments. F, G Comparison of RSP F and RSE G for quantifying colocalization of Nicastrin for functional zones (pre/CAZ, post/PSD, peri/EZ) of an excitatory synapse. The data are represented as mean ± SEM. Significance was determined by one-way analysis of variance (ANOVA) test followed by Tukey’s multiple comparison test. Indications of significance correspond to P values *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001, ns P > 0.05. n = 5155 (pre), 3016 (post) and 3966 puncta (peri) from 3–4 biological repeatsBack to article page