Fig. 3
From: Ackr3-Venus knock-in mouse lights up brain vasculature
Ackr3-Venus knock-in mouse generation. A The scheme shows the strategy used to generate Ackr3-Venus knock-in mice (see methods). Briefly, the intron 1- exon 2 of Ackr3 was replaced with a targeted fragment containing Ackr3-fused to Venus at C-tail. LoxP sites are shown by black flags, closed box is 3′ UTR. B–E Evaluation of Ackr3-Venus transcript levels from RNA extracts of whole mouse brain. Real-time qPCR results show expression levels of the following transcripts Ackr3 (B), Venus (C) mGluR5 (D), and β-actin (E), in Ackr3WT/WT, Ackr3Venus/WT, and Ackr3Venus/Venus (n = 3–6 animals per group). The ΔΔCT in percent of WT is shown for each transcript except Venus where KI was used for normalization. Significance is shown as *p < 0.05 and ***p < 0.001 in a one-way ANOVA with Dunn’s multiple comparison’s test