Figure 3From: Endoplasmic reticulum chaperone GRP78 is involved in autophagy activation induced by ischemic preconditioning in neural cells Suppression of GRP78 with siRNA inhibited autophagy activation and neuroprotection induced by IPC in PC12 cells. (A) GRP78 siRNA1 cancelled the IPC-induced neuroprotection. Cells were transfected with GRP78 siRNA1 (40 nM) or negative control sequence (NC) twenty-four hours before the onset of IPC. Twelve hours after IPC, the cells were subjected to OGD for 10 h and cell viability was examined with CCK-8 kit. (B)-(D) The cells were harvested 12 h after IPC and subjected to Western blot analysis. (B) GRP78 siRNA1 reversed IPC-induced upregulation of GRP78. (C) GRP78 siRNA1 reversed IPC-induced upregulation of LC3II/LC3I. (D) GRP78 siRNA1 reversed IPC-induced upregulation of Beclin1. Bar represents mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group; $$P < 0.01 compared with the OGD group; %% P < 0.01 compared with the NC + IPC + OGD group; # P < 0.05 compared with the NC + IPC group.Back to article page