Fig. 1
From: The neurotoxicity of amyloid β-protein oligomers is reversible in a primary neuron model
Effects of Aβ-O treatment and removal on caspase-3 and eIF2α in primary cortical neurons. a Experimental design. Primary neurons (DIV9) were treated with 2.5 μM Aβ-O (O) or vehicle (C) for 2 days. On day 2, Aβ-O-treated neurons were washed and further treated with Aβ-O (O) or deprived of Aβ-O (O-R) for an additional 2 days. Control neurons (C) were similarly cultured for an additional 2 days. b Cell survival assay. Cell survival was analyzed by CCK-8 assay as described in Methods. The graph shows survival levels relative to those in controls on day 2 or 4. c Cells were lysed on day 2 or 4, and cell lysates were analyzed by Western blotting using an anti-cleaved caspase-3 antibody. d Cleaved caspase-3 levels were quantified and expressed relative to those in control neurons on day 2. e Cell lysates were analyzed by Western blotting using anti-eIF2α or anti-p-eIF2α antibodies. f Total and p-eIF2α levels were quantified and expressed as p-eIF2α/total eIF2α ratios relative to those in control neurons on day 2. Data represent means ± SEM from three separate experiments. *p < 0.05, **p < 0.01, compared with control. #p < 0.05, compared with Aβ-O-treated cells