Test | Controls | Mutants | F value | p value | ||
---|---|---|---|---|---|---|
Mean | SEM | Mean | SEM | |||
General health/neurological screen | ||||||
 body temperature (°C) | 36.85 | 0.141 | 37.28 | 0.164 | 2.747 | 0.1049 |
 grip strength (n) | 0.554 | 0.02 | 0.577 | 0.012 | 1.038 | 0.3141 |
 wire hang (latency to fall, s) | 39.512 | 5.428 | 29.507 | 2.879 | 3.204 | 0.0808 |
Light/dark transition | ||||||
 latency to light (s) | 44.143 | 5.829 | 56.1 | 6.621 | 1.293 | 0.2619 |
 light distance (cm) | 622.129 | 46.667 | 609.563 | 41.119 | 0.034 | 0.8548 |
 dark distance (cm) | 1555.886 | 65.493 | 1628.983 | 35.355 | 1.147 | 0.2904 |
 number of transitions | 32.714 | 2.23 | 28.967 | 1.965 | 1.32 | 0.2571 |
 stay time in light (s) | 158.071 | 9.869 | 147.5 | 10.065 | 0.423 | 0.5189 |
Open field | ||||||
 stereotypic counts | 6911.643 | 520.353 | 7369.367 | 526.983 | 0.289 | 0.5939 |
 vertical activity | 348.357 | 53.683 | 275.4 | 43.994 | 0.966 | 0.3312 |
 center time (s) | 633.986 | 121.934 | 813.077 | 111.923 | 0.945 | 0.3365 |
Elevated plus maze | ||||||
 number of total entry (times) | 36.071 | 2.319 | 36.833 | 2.229 | 0.044 | 0.835 |
 entry into open arms (%) | 13.393 | 2.567 | 13.863 | 1.572 | 0.027 | 0.8715 |
 total distance travelled (cm) | 1806.893 | 92.545 | 1880.087 | 73.559 | 0.343 | 0.5614 |
 time on open arms (times) | 3.443 | 1.125 | 2.923 | 0.744 | 0.152 | 0.6985 |
Hot plate | ||||||
 latency (s) | 6.403 | 0.386 | 6.318 | 0.394 | 0.018 | 0.8944 |
Social interaction (novel environment) | ||||||
 duration of contacts (s) | 68.7 | 9.138 | 88.707 | 7.29 | 2.605 | 0.1222 |
 number or contacts (times) | 40.143 | 3.845 | 45.333 | 2.462 | 1.358 | 0.2577 |
 mean duration of contacts (s) | 1.786 | 0.263 | 1.993 | 0.187 | 0.4 | 0.5341 |
 distance travelled (cm) | 2957.586 | 285.871 | 3196.793 | 153.133 | 0.654 | 0.4283 |
Porsolt forced swim | ||||||
 day1 immobility (%) | 41.8131 | 3.617 | 46.252 | 2.314 | 1.099 | 0.3009 |
 day2 immobility (%) | 57.747 | 3.627 | 54.268 | 2.624 | 0.569 | 0.4551 |
Gait analysis | ||||||
 front swing/stride duration | 41 | 0.508 | 40.969 | 0.43 | 0.002 | 0.9662 |
 front brake/stride duration | 28.115 | 0.909 | 27.234 | 0.715 | 0.512 | 0.4784 |
 front propel/stride duration | 30.896 | 0.871 | 31.795 | 0.645 | 0.635 | 0.4302 |
 front stance width | 1.946 | 0.062 | 1.921 | 0.038 | 0.134 | 0.7164 |
 front step angle | 62.308 | 1.762 | 60.066 | 1.075 | 1.269 | 0.2666 |
 front paw angle | −0.235 | 0.866 | 0.89 | 0.455 | 1.591 | 0.2144 |
 hind brake/stride duration | 14.142 | 0.831 | 15.564 | 0.364 | 3.373 | 0.0737 |
 hind step angle | 53.746 | 1.757 | 55.321 | 1.627 | 0.338 | 0.564 |
 hind paw angle | −0.788 | 0.953 | −0.983 | 0.519 | 0.038 | 0.8471 |
Barnes maze | ||||||
 time spent around target (probe test, 1 day) | 40.538 | 4.748 | 39.143 | 4.067 | 0.042 | 0.8385 |
 time spent around target (probe test, 1 month) | 32.154 | 5.78 | 30.172 | 4.224 | 0.072 | 0.7904 |
 time spent around target (reversal test, 1 day) | 31.077 | 4.118 | 28.214 | 3.227 | 0.27 | 0.6064 |
Cued and contextual fear conditioning | ||||||
 conditioning (freezing, %) | 57.756 | 2.66 | 56.821 | 1.83 | 0.82 | 0.7764 |
 context test, 1 day (freezing, %) | 70.082 | 3.444 | 66.333 | 3.372 | 0.453 | 0.5053 |
 cued test, with tone,1 day (freezing, %) | 89.158 | 3.641 | 88.147 | 2.004 | 0.069 | 0.7945 |
 context test, 30 days (freezing, %) | 79.412 | 5.225 | 79.481 | 3.222 | 0.0001376 | 0.9907 |
 cued test, pretone period, 30 days (freezing, %) | 60.603 | 4.475 | 49.672 | 4.306 | 2.345 | 0.1342 |
 cued test, with tone, 30 days (freezing, %) | 90.183 | 3.079 | 87.86 | 1.944 | 0.425 | 0.5186 |
Tail suspension | ||||||
 immobility (%) | 37.255 | 3.747 | 41.75 | 2.603 | 0.957 | 0.334 |
24Â h home cage monitoring | ||||||
 activity light period (A.U.) | 478,882.065 | 30,477.038 | 670,800.611 | 107,540.908 | 2.948 | 0.1167 |
 activity dark period (A.U.) | 1,033,998.833 | 66,355.759 | 1,385,925 | 191,066.128 | 3.027 | 0.1125 |
 number of particle dark period | 1.175 | 0.02 | 1.196 | 0.022 | 0.482 | 0.5034 |
 | Stranger | Empty or Familiar | t value | p value (paired-t) | ||
Mean | SEM | Mean | SEM | Â | Â | |
Social approach test (Stranger vs Empty) | ||||||
 number of entry around cage (controls) | 9 | 1.043 | 7.714 | 0.952 | 1.178 | 0.26 |
 time spent around cage (mutants) | 209.241 | 12.352 | 137.448 | 10.928 | 0.627 | 0.5359 |
Social novelty preference test (Stranger vs Familiar) | ||||||
 number of entry around cage (controls) | 8.357 | 0.887 | 8.786 | 1.11 | −0.405 | 0.6918 |
 number of entry around cage (mutants) | 9.862 | 0.851 | 10.034 | 0.863 | −0.16 | 0.8742 |
 time spent around cage (controls) | 165.071 | 16.433 | 148.5 | 28.882 | 0.435 | 0.6708 |
 time spent around cage (mutants) | 175.31 | 17.16 | 156.379 | 13.814 | 0.673 | 0.5063 |